《植物生理学报》 2016, 52(4): 479-486

接种枯萎病菌对甜瓜脂氧合酶活性及基因表达的影响

邵琪, 解群杰, 刘贺娟, 齐红岩^*

沈阳农业大学园艺学院, 设施园艺省部共建教育部重点实验室, 辽宁省设施园艺重点实验室, 沈阳 110866

通信作者：齐红岩；E-mail: hyqiaaa@126.com

摘　要：

本文研究薄皮甜瓜脂氧合酶(lipoxygenase, LOXs)及其基因家族成员(CmLOXs)在接种枯萎病后的响应。以薄皮甜瓜‘玉美人’幼苗为试材, “三叶一心”时, 灌根法接种枯萎病菌-尖孢镰刀菌(Fusarium oxysporum f. sp. melonis), 在接种后不同时间测定了幼苗电解质渗透率、根系活力、LOX活性及CmLOXs基因相对表达量。接种枯萎病菌后甜瓜叶片中有13个Cm-LOXs基因在接种枯萎病菌后上调表达。其中除CmLOX02和CmLOX11在第3天显著升高外, 其他11个基因(CmLOX03、Cm-LOX07~10、CmLOX12~17)均在第5天时相对表达量达到最大值。甜瓜根部有5个CmLOXs基因上调表达, 其中CmLOX03、CmLOX16和CmLOX17在处理1 d后有显著升高, CmLOX05和CmLOX06在接种3 d时与对照有显著差异。薄皮甜瓜幼苗在接种枯萎病菌时, 根系活力显著下降, 电解质渗透率升高, 叶片和根中LOX活性升高, 叶片和根中分别有13个和5个CmLOXs成员上调表达, 但响应时间不同, 这些结果表明CmLOXs可能参与防御反应, 本研究为后续通过转基因技术研究其在生物胁迫中的作用奠定基础。

关键词：薄皮甜瓜; 尖孢镰刀菌; 脂氧合酶活性; 基因表达

收稿：2015-08-31   修定：2016-03-15

资助：国家自然科学基金(31272154)。

Effects of LOX activity and gene expression of Cucumis melo var. makuwa inoculated by Fusarium oxysporum

SHAO Qi, XIE Qun-Jie, LIU He-Juan, QI Hong-Yan^*

Key Laboratory of Protected Horticulture of Liaoning Province, Key Laboratory of Protected Horticulture, Ministry of Education, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China

Corresponding author: QI Hong-Yan; E-mail: hyqiaaa@126.com

Abstract:

The effects of lipoxygenase (lipoxygenase, LOXs) and gene family members (CmLOXs) in the oriental melon inoculated with Fusarium oxysporum f. sp. melonis were studied. The oriental melon cultivar ‘Yumeiren’ were used as the experimental materials, they were inoculated with Fusarium oxysporum at the period of three leaves, then the conductivity, root vigor, LOX activity and real-time fluorescent quantitative PCR analysis of CmLOXs gene expressions were determined in different days. There were 13 CmLOXs up-regulated in leaves after inoculating with Fusarium oxysporum f. sp. Melonis. CmLOX02 and CmLOX11 were significantly higher at 3 days after inoculation, while other 11 CmLOXs (CmLOX03, CmLOX07–10, CmLOX12–17) reached peak value at 5 days. Meanwhile, there were 5 CmLOXs up-regulated in roots after inoculation. CmLOX03, CmLOX16 and CmLOX17 were significantly increased at 1 days, CmLOX05 and CmLOX06 were significantly higher than the control at 3 days. In addition, LOX activity and conductivity in leaves and roots increased, while root vigor decreased when melon seedlings infected Fusarium oxysporum. The results suggested that LOX gene family might participate in response and will be helpful in transgenic experiment to understand their precise roles of melon in biotic stress.

Key words: oriental melon (Cucumis melo var. makuwa); Fusarium oxysporum f. sp. melonis; LOX activity; gene expression